• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

EIF2B1 Monoclonal antibody

EIF2B1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6A3

Cat no : 68824-1-Ig

Print datasheet

Synonyms

EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B

验证数据展示

  • WB analysis using 68824-1-Ig

    WB analysis using 68824-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68824-1-Ig

    WB analysis of NIH/3T3 using 68824-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 68824-1-Ig

    IF Staining of HeLa using 68824-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2B1 antibody (68824-1-Ig, Clone: 1H6A3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inNIH/3T3 cells, U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68824-1-Ig targets EIF2B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, pig
Immunogen EIF2B1 fusion protein Ag12493 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Synonyms EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B
Calculated Molecular Weight 305 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14232
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF2B1 antibody 68824-1-IgDownload protocol
IF protocol for EIF2B1 antibody 68824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols