验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse liver tissue, HEK-293 cells, K-562 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11034-1-AP targets EIF2B2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | EIF2B2 fusion protein Ag1502 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa |
| 别名 | EIF 2Bbeta, EIF2B, EIF2B2, EIF2BB, S20I15, S20III15 |
| 计算分子量 | 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC011750 |
| 基因名称 | EIF2B2 |
| Gene ID (NCBI) | 8892 |
| RRID | AB_2096016 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49770 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| IHC protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| IP protocol for EIF2B2 antibody 11034-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. | ||
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
Elife A point mutation in the nucleotide exchange factor eIF2B constitutively activates the integrated stress response by allosteric modulation. | ||
RNA EIF2B2 mutations in Vanishing White Matter Disease hyper- suppress translation and delay recovery during the integrated stress response. | ||
Biochem J Stoichiometry of the eIF2B complex is maintained by mutual stabilization of subunits.
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