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验证数据展示

  • WB analysis using 11296-2-AP

    WB analysis using 11296-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11296-2-AP (EIF2B3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 11296-2-AP

    IHC staining of mouse colon using 11296-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11296-2-AP (EIF2B3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11296-2-AP

    IF Staining of HeLa using 11296-2-AP

    Immunofluorescent analysis of HeLa cells using 11296-2-AP (EIF2B3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

EIF2B3 Polyclonal antibody

EIF2B3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11296-2-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Translation initiation factor eIF2B subunit gamma, EIF2Bgamma, eIF2B GDP-GTP exchange factor subunit gamma, EIF 2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11296-2-AP targets EIF2B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 EIF2B3 fusion protein Ag1809 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
别名 Translation initiation factor eIF2B subunit gamma, EIF2Bgamma, eIF2B GDP-GTP exchange factor subunit gamma, EIF 2B
计算分子量 44 kDa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC018728
基因名称 EIF2B3
Gene ID (NCBI) 8891
RRIDAB_2096142
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR50
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF2B3 antibody 11296-2-APDownload protocol
IHC protocol for EIF2B3 antibody 11296-2-APDownload protocol
IF protocol for EIF2B3 antibody 11296-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Elife

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

Authors - Yao Liang Wong
View Article
humanIF

Mol Biol Cell

Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs.

Authors - Rachel E Hodgson
View Article