- Featured Product
- KD/KO Validated
EIF2S1 Monoclonal antibody
EIF2S1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3C8E2
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HEK-293 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IP detected in | HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
68479-1-Ig targets EIF2S1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human |
Immunogen | EIF2S1 fusion protein Ag28309 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa |
Synonyms | EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC002513 |
Gene Symbol | EIF2S1 |
Gene ID (NCBI) | 1965 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P05198 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for EIF2S1 antibody 68479-1-Ig | Download protocol |
IF protocol for EIF2S1 antibody 68479-1-Ig | Download protocol |
IP protocol for EIF2S1 antibody 68479-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Biosci (Landmark Ed) EIF2Ss, a Novel c-Myc-Correlated Gene Family, is Associated with Poor Prognosis and Immune Infiltration in Pancreatic Adenocarcinoma |