EIF2S2 Monoclonal antibody

EIF2S2 Monoclonal Antibody for WB, ELISA
Cat No. 68463-1-Ig

产品说明书

CloneNo. 2H8E1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

DKFZp686L18198, eIF 2 beta, EIF2, EIF2B, EIF2beta, EIF2S2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68463-1-Ig targets EIF2S2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 EIF2S2 fusion protein Ag18349 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa
别名 DKFZp686L18198, eIF 2 beta, EIF2, EIF2B, EIF2beta, EIF2S2
计算分子量 38 kDa
观测分子量50 kDa
GenBank蛋白编号BC000461
基因名称 EIF2S2
Gene ID (NCBI) 8894
RRIDAB_3085174
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G Magarose purification
UNIPROT IDP20042
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 2 (eIF2) is composed of three subunits, eIF2 alpha, eIF2 beta (EIF2S2), and eIF2 gamma, which are present in equal molar amounts. eIF2 beta plays a central role in the maintenance of what is generally considered a rate-limiting step in mRNA translation. In the early steps of protein synthesis, eIF2 beta binds GTP and Met-tRNA and transfers Met-tRNA to the 40S ribosomal subunit. At the end of the initiation process, GTP bound to eIF2 beta is hydrolyzed to GDP and the eIF2/GDP complex is released from the ribosome. The exchange of GDP bound to eIF2 beta for GTP is a prerequisite to binding Met-tRNA and is mediated by eIF2 beta, which recycles the eIF2 complex for another round of initiation.

实验方案

Product Specific Protocols
WB protocol for EIF2S2 antibody 68463-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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