• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

    WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    IP experiment of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    IP result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    IF Staining of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2S3 antibody (11162-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

EIF2S3 Polyclonal antibody, PBS Only

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11162-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

EIF2G, EIF2, eIF 2gX, eIF 2gA, eIF 2 gamma X

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

11162-1-PBS targets EIF2S3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 EIF2S3 fusion protein Ag1650 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
别名 EIF2G, EIF2, eIF 2gX, eIF 2gA, eIF 2 gamma X
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC019906
基因名称 EIF2S3
Gene ID (NCBI) 1968
RRIDAB_2246331
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP41091
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]