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EIF3A Polyclonal antibody

EIF3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27665-1-AP

Print datasheet

Synonyms

eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32

验证数据展示

  • WB analysis of BxPC-3 using 27665-1-AP

    WB analysis of BxPC-3 using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27665-1-AP

    IF Staining of HepG2 using 27665-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 27665-1-AP (EIF3A antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 27665-1-AP

    WB analysis using 27665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27665-1-AP (EIF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 27665-1-AP

    IHC staining of human lung cancer using 27665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27665-1-AP (EIF3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3A antibody included

经过测试的应用

Positive WB detected inBxPC-3 cells, SKOV-3 cells, mouse brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3A Ready-To-Use IHC Kit. EIF3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27665-1-AP targets EIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen EIF3A fusion protein Ag24752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit A
Synonyms eIF 3 theta, EIF3, eIF3 p167, eIF3 p170, eIF3 p180, eIF3 p185, eIF3 theta, EIF3A, EIF3S10, KIAA0139, P167, p180, p185, TIF32
Calculated Molecular Weight 1382 aa, 166 kDa
Observed Molecular Weight 166 kDa
GenBank Accession NumberBC114429
Gene Symbol EIF3A
Gene ID (NCBI) 8661
RRIDAB_2880942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14152
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3A antibody 27665-1-APDownload protocol
IHC protocol for EIF3A antibody 27665-1-APDownload protocol
IF protocol for EIF3A antibody 27665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols