• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

EIF3C Polyclonal antibody

EIF3C Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12733-1-AP

Print datasheet

Synonyms

EIF3S8, EIF3CL, eIF3 p110

验证数据展示

  • WB analysis of K-562 using 12733-1-AP

    WB analysis of K-562 using 12733-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 12733-1-AP

    IP experiment of K-562 using 12733-1-AP

    IP result of anti-EIF3C (IP:12733-1-AP, 4ug; Detection:12733-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IHC staining of human stomach cancer using 12733-1-AP

    IHC staining of human stomach cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 12733-1-AP (EIF3C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3C antibody included

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF3C Ready-To-Use IHC Kit. EIF3C primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12733-1-AP targets EIF3C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EIF3C fusion protein Ag3438 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit C
Synonyms EIF3S8, EIF3CL, eIF3 p110
Calculated Molecular Weight 913 aa, 110 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC001571
Gene Symbol EIF3C
Gene ID (NCBI) 8663
RRIDAB_2096747
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3C antibody 12733-1-APDownload protocol
IHC protocol for EIF3C antibody 12733-1-APDownload protocol
IP protocol for EIF3C antibody 12733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Differ

Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression

Authors - Yanan Pi
View Article