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EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10899-1-AP

Print datasheet

Synonyms

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

验证数据展示

  • WB analysis of rat lymph using 10899-1-AP

    WB analysis of rat lymph using 10899-1-AP

    rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 10899-1-AP

    IP experiment of Jurkat using 10899-1-AP

    IP result of anti-EIF3E (IP:10899-1-AP, 4ug; Detection:10899-1-AP 1:500) with Jurkat cells lysate 2440 ug.

  • IHC staining of human pancreas cancer using 10899-1-AP

    IHC staining of human pancreas cancer using 10899-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with EIF3E antibody included
  • IF Staining of SH-SY5Y using 10899-1-AP

    IF Staining of SH-SY5Y using 10899-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 10899-1-AP

    IF Staining of U2OS using 10899-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inrat lymph tissue, HeLa cells, mouse thymus tissue, mouse pancreas tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10899-1-AP targets EIF3E in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EIF3E fusion protein Ag1132 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit E
Synonyms Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48
Calculated Molecular Weight 445 aa, 52 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC008419
Gene Symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2096898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60228
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3E antibody 10899-1-APDownload protocol
IHC protocol for EIF3E antibody 10899-1-APDownload protocol
IF protocol for EIF3E antibody 10899-1-APDownload protocol
IP protocol for EIF3E antibody 10899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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