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验证数据展示

  • IP experiment of HeLa using 67095-1-Ig

    IP experiment of HeLa using 67095-1-Ig

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug.

  • WB analysis using 67095-1-Ig

    WB analysis using 67095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67095-1-Ig

    IF Staining of HepG2 using 67095-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

EIF3E Monoclonal antibody

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 67095-1-Ig

Print datasheet

CloneNo. 1C6C8
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Synonyms

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48, 1C6C8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1800 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

RIPSee 1 publications below

产品信息

67095-1-Ig targets EIF3E in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsRIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen EIF3E fusion protein Ag28523 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit E
Synonyms Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48, 1C6C8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberBC000734
Gene Symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60228
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3E antibody 67095-1-IgDownload protocol
IF protocol for EIF3E antibody 67095-1-IgDownload protocol
IP protocol for EIF3E antibody 67095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Cancer Res

Extracellular Vesicles Containing circMYBL1 Induce CD44 in Adenoid Cystic Carcinoma Cells and Pulmonary Endothelial Cells to Promote Lung Metastasis

Authors - Min Fu
View Article