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验证数据展示

  • IP experiment of HeLa using 67095-1-Ig

    IP experiment of HeLa using 67095-1-Ig

    IP result of anti-EIF3E (IP:67095-1-Ig, 5ug; Detection:67095-1-Ig 1:2000) with HeLa cells lysate 1600 ug.

  • WB analysis using 67095-1-Ig

    WB analysis using 67095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67095-1-Ig (EIF3E antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67095-1-Ig

    IF Staining of HepG2 using 67095-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF3E antibody (67095-1-Ig, Clone: 1C6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

EIF3E Monoclonal antibody

EIF3E Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 67095-1-Ig

产品说明书

CloneNo. 1C6C8
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48, 1C6C8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

RIPSee 1 publications below

产品信息

67095-1-Ig targets EIF3E in WB, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用RIP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 EIF3E fusion protein Ag28523 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit E
别名 Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48, 1C6C8
计算分子量 48 kDa
观测分子量 48-52 kDa
GenBank蛋白编号BC000734
基因名称 EIF3E
Gene ID (NCBI) 3646
RRIDAB_2882400
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP60228
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3E antibody 67095-1-IgDownload protocol
IF protocol for EIF3E antibody 67095-1-IgDownload protocol
IP protocol for EIF3E antibody 67095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Cancer Res

Extracellular Vesicles Containing circMYBL1 Induce CD44 in Adenoid Cystic Carcinoma Cells and Pulmonary Endothelial Cells to Promote Lung Metastasis

Authors - Min Fu
View Article