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验证数据展示

  • WB analysis using 84131-2-RR (same clone as 84131-2-PBS)

    WB analysis using 84131-2-RR (same clone as 84131-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84131-2-RR (EIF3F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01038-4

    Sandwich ELISA standard curve of MP01038-4

    Sandwich ELISA standard curve of MP01038-4, Human EIF3F Recombinant Matched Antibody Pair - PBS only. 84131-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34849. 84131-2-PBS was HRP conjugated as the detection antibody. Range: 0.469-30 ng/mL.

  • Sample test of MP01038-4

    Sample test of MP01038-4

    The mean EIF3F concentration was determined to be 225.37 ng/mL in Jurkat cell extract based on a 1.60 mg/mL extract load.

  • IF Staining of HeLa using 84131-2-RR (same clone as 84131-2-PBS)

    IF Staining of HeLa using 84131-2-RR (same clone as 84131-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3F antibody (84131-2-RR, Clone: 240894D2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84131-2-RR (same clone as 84131-2-PBS)

    FC experiment of HeLa using 84131-2-RR (same clone as 84131-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EIF3F Recombinant antibody (84131-2-RR, Clone:240894D2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84131-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01038-1

    Cytometric bead array standard curve of MP01038-1

    Cytometric bead array standard curve of MP01038-1, EIF3F Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84131-2-PBS. Detection antibody: 84131-1-PBS. Standard: Ag34849. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01038-2

    Cytometric bead array standard curve of MP01038-2

    Cytometric bead array standard curve of MP01038-2, EIF3F Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84131-2-PBS. Detection antibody: 84131-3-PBS. Standard: Ag34849. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84131-2-RR

    Affinity and Kinetic Characterization of 84131-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84131-2-RR against Human EIF3F were performed. The affinity constant is 1.78 nM.

EIF3F Recombinant antibody, PBS Only (Capture/Detector)

EIF3F Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84131-2-PBS

产品说明书

CloneNo. 240894D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

eIF3 p47, 240894D2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84131-2-PBS targets EIF3F as part of a matched antibody pair:

MP01038-1: 84131-2-PBS capture and 84131-1-PBS detection (validated in Cytometric bead array)

MP01038-2: 84131-2-PBS capture and 84131-3-PBS detection (validated in Cytometric bead array)

MP01038-4: 84131-1-PBS capture and 84131-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 EIF3F fusion protein Ag34849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit F
别名 eIF3 p47, 240894D2
计算分子量 38 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC000490
基因名称 EIF3F
Gene ID (NCBI) 8665
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00303
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF3F (eukaryotic translation initiation factor 3 subunit F) is a protein that plays an important role in eukaryotic cells and is an integral part of the translation initiation factor 3(Eif3) complex. EIF3 is a complex of at least 10 distinct subunits and is the largest translation initiation factor in eukaryotes.