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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

    WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3M antibody included
  • IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3M antibody included
  • IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF3M antibody (66025-1-Ig, Clone: 2A12B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

EIF3M Monoclonal antibody, PBS Only

EIF3M Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66025-1-PBS

产品说明书

CloneNo. 2A12B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PCID1, HFLB5, hfl B5, GA17, EIF3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF3M Ready-To-Use IHC Kit. EIF3M primary antibody included.

产品信息

66025-1-PBS targets EIF3M in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF3M fusion protein Ag17965 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit M
别名 PCID1, HFLB5, hfl B5, GA17, EIF3
计算分子量 374 aa, 43 kDa
观测分子量 35-43 kDa
GenBank蛋白编号BC019103
基因名称 EIF3M
Gene ID (NCBI) 10480
RRIDAB_11042448
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L2H7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.