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EIF3M Monoclonal antibody, PBS Only

EIF3M Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A12B2

Cat no : 66025-1-PBS

Print datasheet

Synonyms

PCID1, HFLB5, hfl B5, GA17, EIF3

验证数据展示

  • WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

    WB analysis using 66025-1-Ig (same clone as 66025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66025-1-Ig (EIF3M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3M antibody included
  • IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    IHC staining of human colon cancer using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66025-1-Ig (EIF3M antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3M antibody included
  • IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    IF Staining of MCF-7 using 66025-1-Ig (same clone as 66025-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF3M antibody (66025-1-Ig, Clone: 2A12B2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66025-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EIF3M Ready-To-Use IHC Kit. EIF3M primary antibody included.

产品信息

66025-1-PBS targets EIF3M in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EIF3M fusion protein Ag17965 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit M
Synonyms PCID1, HFLB5, hfl B5, GA17, EIF3
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 35-43 kDa
GenBank Accession NumberBC019103
Gene Symbol EIF3M
Gene ID (NCBI) 10480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L2H7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.