验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse ovary tissue, Raji cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human breast cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11280-1-AP targets EIF4A2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | EIF4A2 fusion protein Ag1810 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 4A, isoform 2 |
| 别名 | BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F |
| 计算分子量 | 47 kDa |
| 观测分子量 | 47 kDa |
| GenBank蛋白编号 | BC015842 |
| 基因名称 | EIF4A2 |
| Gene ID (NCBI) | 1974 |
| RRID | AB_2097378 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14240 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF4A2 antibody 11280-1-AP | Download protocol |
| IHC protocol for EIF4A2 antibody 11280-1-AP | Download protocol |
| IP protocol for EIF4A2 antibody 11280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. | ||
Mol Neurobiol Identification of Immune-Related Biomarkers of Schizophrenia in the Central Nervous System Using Bioinformatic Methods and Machine Learning Algorithms | ||
Stem Cell Rev Rep Hyaluronan-CD44 Interaction Regulates Mouse Retinal Progenitor Cells Migration, Proliferation and Neuronal Differentiation | ||
Mol Carcinog miR-181a/b Induces the Growth, Invasion and Metastasis of Neuroblastoma Cells Through Targeting ABI1. | ||
Biol Reprod Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice. |
