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EIF4A3 Polyclonal antibody, PBS Only

EIF4A3 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10463-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.

产品信息

10463-1-PBS targets EIF4A3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen EIF4A3 fusion protein Ag0733 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 3
Synonyms
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC004386
Gene Symbol EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2097394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38919
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions