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EIF4A3 Monoclonal antibody, PBS Only

EIF4A3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G6H9

Cat No : 67740-1-PBS

Print datasheet

Synonyms

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

验证数据展示

  • WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

    IHC staining of human colon cancer using 67740-1-Ig (same clone as 67740-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67740-1-Ig (EIF4A3 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4A3 antibody included
  • WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    WB analysis using 67740-1-Ig (same clone as 67740-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67740-1-Ig (EIF4A3 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67740-1-Ig (same clone as 67740-1-PBS)

    IF Staining of HepG2 using 67740-1-Ig (same clone as 67740-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EIF4A3 antibody (67740-1-Ig, Clone: 1G6H9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67740-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.

产品信息

67740-1-PBS targets EIF4A3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen EIF4A3 fusion protein Ag11130 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 3
Synonyms DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC003662
Gene Symbol EIF4A3
Gene ID (NCBI) 9775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38919
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions