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EIF4E Polyclonal antibody

EIF4E Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29712-1-AP

Print datasheet

Synonyms

CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein

验证数据展示

  • WB analysis using 29712-1-AP

    WB analysis using 29712-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29712-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 29712-1-AP

    IF Staining of HepG2 using 29712-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (29712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of human colon cancer using 29712-1-AP

    IHC staining of human colon cancer using 29712-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF4E antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HSC-T6 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29712-1-AP targets EIF4E in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E
Synonyms CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F, mRNA cap binding protein
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.

实验方案

Product Specific Protocols
WB protocol for EIF4E antibody 29712-1-APDownload protocol
IHC protocol for EIF4E antibody 29712-1-APDownload protocol
IF protocol for EIF4E antibody 29712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols