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EIF4E3 Monoclonal antibody

EIF4E3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E9C4

Cat no : 68171-1-Ig

Print datasheet

Synonyms

eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3

验证数据展示

  • WB analysis using 68171-1-Ig

    WB analysis using 68171-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68171-1-Ig (EIF4E3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse colon using 68171-1-Ig

    IHC staining of mouse colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 68171-1-Ig

    IHC staining of mouse colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 68171-1-Ig

    IHC staining of rat colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 68171-1-Ig

    IHC staining of rat colon using 68171-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68171-1-Ig (EIF4E3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, MOLT-4 cells, Jurkat cells, THP-1 cells, K-562 cells
Positive IHC detected inrat colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68171-1-Ig targets EIF4E3 in WB, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen EIF4E3 fusion protein Ag11056 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E family member 3
Synonyms eIF 4E type 3, eIF 4E3, eIF4E 3, eIF4E type 3, EIF4E3
Calculated Molecular Weight 24 kDa
Observed Molecular Weight24-30 kDa
GenBank Accession NumberBC031289
Gene Symbol EIF4E3
Gene ID (NCBI) 317649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N5X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4E3, also known as Eukaryotic translation initiation factor 4E type 3, is a 224 amino acid protein, which recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. EIF4E3 acts as a tissue-specific tumor suppressor. For instance, eIF4E3 inhibits expression of both mRNA export and translation targets of eIF4E1, consistent with its nuclear and cytoplasmic localization. Importantly, eIF4E3 overexpression represses oncogenic transformation. (PMID: 23587918).

实验方案

Product Specific Protocols
WB protocol for EIF4E3 antibody 68171-1-IgDownload protocol
IHC protocol for EIF4E3 antibody 68171-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols