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验证数据展示

  • IHC staining of human prostate cancer using 11182-1-AP

    IHC staining of human prostate cancer using 11182-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11182-1-AP (EIF4EBP2 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of mouse testis using 11182-1-AP

    WB analysis of mouse testis using 11182-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11182-1-AP (EIF4EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

EIF4EBP2 Polyclonal antibody

EIF4EBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11182-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11182-1-AP targets EIF4EBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitypig
Immunogen EIF4EBP2 fusion protein Ag1670 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E binding protein 2
Synonyms Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2
Calculated Molecular Weight 13 kDa
Observed Molecular Weight ~20 kDa
GenBank Accession NumberBC050633
Gene Symbol EIF4EBP2
Gene ID (NCBI) 1979
RRIDAB_2877750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13542
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
IHC protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Cell Mol Life Sci

Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs

Authors - Xiaolong Yuan
View Article