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验证数据展示

  • IHC staining of human prostate cancer using 11182-1-AP

    IHC staining of human prostate cancer using 11182-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11182-1-AP (EIF4EBP2 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of mouse testis using 11182-1-AP

    WB analysis of mouse testis using 11182-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11182-1-AP (EIF4EBP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

EIF4EBP2 Polyclonal antibody

EIF4EBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11182-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11182-1-AP targets EIF4EBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性pig
免疫原 EIF4EBP2 fusion protein Ag1670 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 2
别名 Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2
计算分子量 13 kDa
观测分子量 ~20 kDa
GenBank蛋白编号BC050633
基因名称 EIF4EBP2
Gene ID (NCBI) 1979
RRIDAB_2877750
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13542
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
IHC protocol for EIF4EBP2 antibody 11182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Cell Mol Life Sci

Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs

Authors - Xiaolong Yuan
View Article