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验证数据展示

  • WB analysis using 15628-1-AP

    WB analysis using 15628-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15628-1-AP (EIF4EBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

EIF4EBP2 Polyclonal antibody

EIF4EBP2 Polyclonal Antibody for WB, ELISA
Cat No. 15628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15628-1-AP targets EIF4EBP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF4EBP2 fusion protein Ag8101 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 2
别名 Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2, eIF4E binding protein 2, 4E-BP2, 4EBP2
计算分子量 120 aa, 13 kDa
观测分子量 15-20 kDa
GenBank蛋白编号BC005057
基因名称 EIF4EBP2
Gene ID (NCBI) 1979
RRIDAB_3669215
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13542
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4EBP2(Eukaryotic translation initiation factor 4E-binding protein 2), also named as 4EBP2, is a member of the eukaryotic translation initiation factor 4E binding protein family. EIF4EBP2 is enriched in brain and acts as a regulator of synapse activity and neuronal stem cell renewal via its ability to repress translation initiation. EIF4EBP2 is a repressor of translation initiation involved in synaptic plasticity, learning and memory formation (PubMed:30765518).

实验方案

Product Specific Protocols
WB protocol for EIF4EBP2 antibody 15628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols