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EIF4G1 Monoclonal antibody, PBS Only

EIF4G1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B10G8

Cat No : 67199-1-PBS

Print datasheet

Synonyms

DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220

验证数据展示

  • WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

    WB analysis using 67199-1-Ig (same clone as 67199-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67199-1-Ig (EIF4G1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

    IF Staining of HepG2 using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4G1 antibody included
  • IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    IHC staining of mouse pancreas using 67199-1-Ig (same clone as 67199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67199-1-Ig (EIF4G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4G1 antibody included
Planning an IHC experiment? We recommend our IHCeasy EIF4G1 Ready-To-Use IHC Kit. EIF4G1 primary antibody included.

产品信息

67199-1-PBS targets EIF4G1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen EIF4G1 fusion protein Ag8357 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 1
Synonyms DKFZp686A1451, eIF 4 gamma 1, eIF 4G 1, eIF 4G1, EIF4F, EIF4G, EIF4G1, EIF4GI, p220
Calculated Molecular Weight 1600 aa, 176 kDa
Observed Molecular Weight 240-250 kDa
GenBank Accession NumberBC007788
Gene Symbol EIF4G1
Gene ID (NCBI) 1981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04637
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )