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eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat No : 17728-1-AP

Print datasheet

Synonyms

AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, NAT1, p97

验证数据展示

  • WB analysis using 17728-1-AP

    WB analysis using 17728-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 17728-1-AP

    IP experiment of MCF-7 using 17728-1-AP

    IP result of anti-eIF4G2/DAP5 (IP:17728-1-AP, 4ug; Detection:17728-1-AP 1:400) with MCF-7 cells lysate 3200ug.

  • IHC staining of human prostate cancer using 17728-1-AP

    IHC staining of human prostate cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF4G2 antibody included
  • IHC staining of human prostate cancer using 17728-1-AP

    IHC staining of human prostate cancer using 17728-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF4G2 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, Neuro-2a cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EIF4G2 Ready-To-Use IHC Kit. EIF4G2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17728-1-AP targets eIF4G2/DAP5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen eIF4G2/DAP5 fusion protein Ag12125 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4 gamma, 2
Synonyms AAG1, DAP 5, DAP5, Death associated protein 5, eIF 4 gamma 2, eIF 4G 2, eIF4G 2, eIF4G2, NAT1, p97
Calculated Molecular Weight 907 aa, 102 kDa
Observed Molecular Weight 98-102 kDa
GenBank Accession NumberBC043149
Gene Symbol EIF4G2
Gene ID (NCBI) 1982
RRIDAB_2095895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78344
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IHC protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
IP protocol for eIF4G2/DAP5 antibody 17728-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

PMID: 31792210

Authors - Sunwang Xu
View Article
humanWB

Shock

miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2

Authors - Zirui Jia
View Article