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验证数据展示

  • WB analysis using 11281-1-AP

    WB analysis using 11281-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 11281-1-AP

    IHC staining of human gliomas using 11281-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens).

  • IP experiment of HeLa using 11281-1-AP

    IP experiment of HeLa using 11281-1-AP

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug.

  • IF Staining of HeLa using 11281-1-AP

    IF Staining of HeLa using 11281-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EIF4G3 Polyclonal antibody

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11281-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11281-1-AP targets EIF4G3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 EIF4G3 fusion protein Ag1811 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4 gamma, 3
别名 eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3
计算分子量 177 kDa
观测分子量 70 kDa, 250 kDa
GenBank蛋白编号BC030578
基因名称 EIF4G3
Gene ID (NCBI) 8672
RRIDAB_2877754
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43432
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for EIF4G3 antibody 11281-1-APDownload protocol
IHC protocol for EIF4G3 antibody 11281-1-APDownload protocol
IF protocol for EIF4G3 antibody 11281-1-APDownload protocol
IP protocol for EIF4G3 antibody 11281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Jpn J Clin Oncol

Identification of distinct genomic features reveals frequent somatic AHNAK and PTEN mutations predominantly in primary malignant melanoma presenting in the ureter.

Authors - Yan Huang
View Article
humanWB

Cancer Res

Fatty Acid Oxidation Mediated by Malonyl-CoA Decarboxylase Represses Renal Cell Carcinoma Progression

Authors - Lijie Zhou
View Article
humanIF

Sci Adv

High-throughput tagging of endogenous loci for rapid characterization of protein function

Authors - Joonwon Kim
View Article