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EIF4H Polyclonal antibody
EIF4H Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, PC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
11012-1-AP targets EIF4H in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | EIF4H fusion protein Ag1468 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 4H |
| Synonyms | eIF 4H, EIF4H, KIAA0038, WBSCR1, WSCR1 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC010021 |
| Gene Symbol | EIF4H |
| Gene ID (NCBI) | 7458 |
| RRID | AB_2096035 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15056 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
eukaryotic initiation factor (eIF)4H, which encoded by the gene for HUMORFU_1, functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. And its homolog in human, WBSCR1, located on 7q11, is commonly missed in patients suffered the Williams-Beuren syndrome, which is a complex neurodevelopmental disorder characterized by cardiovascular defects, cerebral dysplasias and a peculiar cognitive-behavioral profile. EIF4H has two kind of isoforms. The short isoform(~25kDa) is the predominant isoform and is expressed alone in liver and skeletal muscle. This is a rabbit anti-EIF4H polyclonal antibody raised against part of N-terminal chain of human EIF4H, and can recognize two kinds of isoform.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF4H antibody 11012-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Virol Small interfering RNAs that deplete the cellular translation factor eIF4H impede mRNA degradation by the virion host shutoff protein of herpes simplex virus.
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