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EIF5A1/EIF5A2 Polyclonal antibody
EIF5A1/EIF5A2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, A2780 cells, mouse testis tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17069-1-AP targets EIF5A1/EIF5A2 in WB, IHC, IP, RIP, ELISA, IF applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IP, RIP, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | EIF5A1/EIF5A2 fusion protein Ag10895 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 5A2 |
Synonyms | eIF 5A 2, EIF 5A2, EIF5A1/EIF5A2, EIF5A2, eIF5AII |
Calculated Molecular Weight | 153 aa, 17 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC036072 |
Gene Symbol | EIF5A2 |
Gene ID (NCBI) | 56648 |
RRID | AB_2262009 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9GZV4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for EIF5A1/EIF5A2 antibody 17069-1-AP | Download protocol |
IHC protocol for EIF5A1/EIF5A2 antibody 17069-1-AP | Download protocol |
IP protocol for EIF5A1/EIF5A2 antibody 17069-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Oncogene Identification of genes that promote PI3K pathway activation and prostate tumour formation | ||
PLoS Biol Klf5 down-regulation induces vascular senescence through eIF5a depletion and mitochondrial fission. | ||
FASEB J Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain. | ||
PLoS One Novel functional MAR elements of double minute chromosomes in human ovarian cells capable of enhancing gene expression. | ||