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ELAC1 Polyclonal antibody

ELAC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26639-1-AP

Print datasheet

Synonyms

D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1

验证数据展示

  • IHC staining of human liver cancer using 26639-1-AP

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 26639-1-AP

    IHC staining of human liver cancer using 26639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26639-1-AP (ELAC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse liver using 26639-1-AP

    WB analysis of mouse liver using 26639-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26639-1-AP (ELAC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26639-1-AP targets ELAC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ELAC1 fusion protein Ag23939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elaC homolog 1 (E. coli)
Synonyms D29, Deleted in Ma29, elaC homolog 1 (E. coli), ElaC homolog protein 1, ELAC1, Ribonuclease Z 1, RNase Z 1, tRNA 3 endonuclease 1, tRNase Z 1
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC014624
Gene Symbol ELAC1
Gene ID (NCBI) 55520
RRIDAB_2880586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H777
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ELAC1 antibody 26639-1-APDownload protocol
IHC protocol for ELAC1 antibody 26639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols