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ELAVL2 Polyclonal antibody

ELAVL2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14008-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IP, ELISA and More (3)

HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse cerebellum tissue
Positive IP detected inmouse cerebellum tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14008-1-AP targets ELAVL2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, chicken
免疫原 ELAVL2 fusion protein Ag5105 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
别名 HUB, Hu-antigen B, ELAV-like protein 2, ELAV-like neuronal protein 1, ELAVL 2
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC030692
基因名称 ELAVL2
Gene ID (NCBI) 1993
RRIDAB_2096356
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12926
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].

实验方案

Product Specific Protocols
WB protocol for ELAVL2 antibody 14008-1-APDownload protocol
IHC protocol for ELAVL2 antibody 14008-1-APDownload protocol
IP protocol for ELAVL2 antibody 14008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages

Authors - Teodorus Theo Susanto
mouseIP

Sci Adv

Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development

Authors - Yuki Takada
humanWB,IF,RIP

Nucleic Acids Res

HuB and HuD repress telomerase activity by dissociating HuR from TERC.

Authors - Xiaolei Cheng
  • KD Validated
mouseRIP

EMBO Rep

ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles.

Authors - Yuzuru Kato
chickenIF

Cell Rep

miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability.

Authors - Ronald J Parchem
humanWB

Hum Mol Genet

ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.

Authors - Stefano Berto
  • KD Validated
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