验证数据展示
经过测试的应用
| Positive WB detected in | human cerebellum tissue, mouse cerebellum tissue, PC-13 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13032-1-AP targets HuA/B/C/D in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | HuA/B/C/D fusion protein Ag3678 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| 别名 | HUD, ELAVL4, ELAV like protein 4, Hu-antigen D, Hu C/D |
| 计算分子量 | 380 aa, 42 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC036071 |
| 基因名称 | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2096385 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P26378 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Embryonic lethal, abnormal vision, Drosophila-like 4 (ELAVL4) is a human homologue of the Drosophilia gene ELAV, which is implicated in neuronal differentiation and maintenance. The ELAVL proteins are mRNA binding proteins and all members of the family contain three RNA-recognition motifs [PMID:12402251]. ELAVL-proteins also interact with inflammation-associated factors. The protein may be implicated in neurodegeneration through these complex mechanisms [PMID:15827745].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IHC protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IF protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| IP protocol for HuA/B/C/D antibody 13032-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Neurosci Methods Efficient infection of organotypic hippocampal slice cultures with adenovirus carrying the transgene REST/NRSF. | ||
J Cosmet Dermatol Study on the correlation of high expression level of HuD with seborrheic keratosis and skin aging | ||
Med Mol Morphol Altered microbiota caused by disordered gut motility leads to an overactivation of intestinal immune system in APC1638T mice | ||
Biomed Pharmacother Shouhui Tongbian Capsule ameliorates constipation via gut microbiota-5-HT-intestinal motility axis |
