验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, human brain tissue, human liver tissue |
Positive IP detected in | A2780 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 3 publications below |
产品信息
12499-1-AP targets ELF2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, goat |
免疫原 | ELF2 fusion protein Ag3170 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | E74-like factor 2 (ets domain transcription factor) |
别名 | E74 like factor 2, ELF2, ELF2A, EU32, NERF, NERF 1A, NERF 1a,b, NERF 1B, NERF 2, New ETS related factor |
计算分子量 | 533 aa, 57 kDa |
观测分子量 | 63-64 kDa, 56-57 kDa |
GenBank蛋白编号 | BC034951 |
基因名称 | ELF2 |
Gene ID (NCBI) | 1998 |
RRID | AB_10642956 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15723 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ELF2 antibody 12499-1-AP | Download protocol |
IP protocol for ELF2 antibody 12499-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Dev Biol A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.
| ||
Cell Biol Int Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2. | ||
Ren Fail ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte. | ||
medRxiv Allelic effects on KLHL17 expression likely mediated by JunB/D underlie a PDAC GWAS signal at chr1p36.33 |