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ELF5 Polyclonal antibody

ELF5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13107-1-AP

Print datasheet

Synonyms

E74 like factor 5, ELF5, ESE 2, ESE2

验证数据展示

  • WB analysis of T-47D using 13107-1-AP

    WB analysis of T-47D using 13107-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 13107-1-AP (ELF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inT-47D cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13107-1-AP targets ELF5 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ELF5 fusion protein Ag3762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name E74-like factor 5 (ets domain transcription factor)
Synonyms E74 like factor 5, ELF5, ESE 2, ESE2
Calculated Molecular Weight 255 aa, 30 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC029743
Gene Symbol ELF5
Gene ID (NCBI) 2001
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKW6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELF5 is an epithelium-specific ETS family transcription factor. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.

实验方案

Product Specific Protocols
WB protocol for ELF5 antibody 13107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols