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验证数据展示

  • WB analysis of T-47D using 13107-1-AP

    WB analysis of T-47D using 13107-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 13107-1-AP (ELF5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ELF5 Polyclonal antibody

ELF5 Polyclonal Antibody for WB, ELISA
Cat No. 13107-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

E74 like factor 5, ELF5, ESE 2, ESE2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13107-1-AP targets ELF5 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ELF5 fusion protein Ag3762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 E74-like factor 5 (ets domain transcription factor)
别名 E74 like factor 5, ELF5, ESE 2, ESE2
计算分子量 255 aa, 30 kDa
观测分子量31 kDa
GenBank蛋白编号BC029743
基因名称 ELF5
Gene ID (NCBI) 2001
RRIDAB_3669152
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKW6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELF5 is an epithelium-specific ETS family transcription factor. In addition to its role in regulating the later stages of terminal differentiation of keratinocytes, it appears to regulate a number of epithelium-specific genes found in tissues containing glandular epithelium such as salivary gland and prostate. It has very low affinity to DNA due to its negative regulatory domain at the amino terminus.

实验方案

Product Specific Protocols
WB protocol for ELF5 antibody 13107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols