验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27420-1-AP targets ELK1 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ELK1 fusion protein Ag26683 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ELK1, member of ETS oncogene family |
| 别名 | ELK , ELK1 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | BC056150 |
| 基因名称 | ELK1 |
| Gene ID (NCBI) | 2002 |
| RRID | AB_2880867 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19419 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELK1 antibody 27420-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation | ||
Front Oncol tRNA-Derived Fragment tRF-Glu-TTC-027 Regulates the Progression of Gastric Carcinoma via MAPK Signaling Pathway | ||
J Transl Med The SETD8/ELK1/bach1 complex regulates hyperglycaemia-mediated EndMT in diabetic nephropathy. | ||
Biochem Biophys Res Commun CircRNA circ-0038718 promotes hepatocellular carcinoma progression through sponging miR-139-3p. | ||
Genes Genomics Tumor-suppressive miRNA-135a inhibits breast cancer cell proliferation by targeting ELK1 and ELK3 oncogenes.
| ||
Cancer Res Commun Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer
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