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验证数据展示

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 21070-1-AP

    WB analysis using 21070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21070-1-AP (ELOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ELOF1 Polyclonal antibody

ELOF1 Polyclonal Antibody for WB, ELISA
Cat No. 21070-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ELF1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, HEK-293 cells, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21070-1-AP targets ELOF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ELOF1 fusion protein Ag15213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elongation factor 1 homolog (S. cerevisiae)
Synonyms ELF1
Calculated Molecular Weight9.5 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC007516
Gene Symbol ELOF1
Gene ID (NCBI) 84337
RRIDAB_10693632
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP60002
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELOF1 (elongation factor 1 homologue) is a small (83 aa), evolutionarily conserved, RNAPII-associated transcription elongation factor that is also a core component of the transcription-coupled DNA repair machinery with an additional role in preventing DNA damage during DNA replication (PMID: 34108663). Loss of ELOF1 strongly compromises AID-mediated deamination, SHM, and CSR, and that this is accompanied by a decrease in parameters of RNAPII pausing/stalling and in RNAPII RNA synthesis without a decrease in levels of S2P-RNAPII (PMID: 39386505).

实验方案

Product Specific Protocols
WB protocol for ELOF1 antibody 21070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols