验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, A431 cells, mouse brain tissue, Y79 cells |
| Positive IHC detected in | human gliomas tissue, human small intestine tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
产品信息
55023-1-AP targets ELOVL4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4 |
| 别名 | STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD |
| 计算分子量 | 37 kDa |
| 观测分子量 | 37-40 kDa |
| GenBank蛋白编号 | NM_022726 |
| 基因名称 | ELOVL4 |
| Gene ID (NCBI) | 6785 |
| RRID | AB_10859792 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9GZR5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELOVL4 antibody 55023-1-AP | Download protocol |
| IHC protocol for ELOVL4 antibody 55023-1-AP | Download protocol |
| IF protocol for ELOVL4 antibody 55023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling
| ||
Ocul Surf PPARγ regulates meibocyte differentiation and lipid synthesis of cultured human meibomian gland epithelial cells (hMGEC). | ||
Int J Mol Sci In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism. | ||
Invest Ophthalmol Vis Sci Isotretinoin Impairs the Secretory Function of Meibomian Gland Via the PPARγ Signaling Pathway. | ||
Lipids Phytosphingosine Increases Biosynthesis of Phytoceramide by Uniquely Stimulating the Expression of Dihydroceramide C4-desaturase (DES2) in Cultured Human Keratinocytes. |
