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ELP3 Polyclonal antibody

ELP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17016-1-AP

Print datasheet

Synonyms

Elongator complex protein 3, ELP3, hELP3, KAT9

验证数据展示

  • WB analysis using 17016-1-AP

    WB analysis using 17016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17016-1-AP (ELP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17016-1-AP

    IHC staining of mouse brain using 17016-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17016-1-AP (ELP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17016-1-AP

    IHC staining of mouse brain using 17016-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17016-1-AP (ELP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17016-1-AP targets ELP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ELP3 fusion protein Ag9993 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elongation protein 3 homolog (S. cerevisiae)
Synonyms Elongator complex protein 3, ELP3, hELP3, KAT9
Calculated Molecular Weight 62 kDa
Observed Molecular Weight60-62 kDa
GenBank Accession NumberBC001240
Gene Symbol ELP3
Gene ID (NCBI) 55140
RRIDAB_2918048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9T3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ELP3 antibody 17016-1-APDownload protocol
IHC protocol for ELP3 antibody 17016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

TDP-43 nuclear loss in FTD/ALS causes widespread alternative polyadenylation changes

Authors - Yi Zeng
View Article
humanIHC

iScience

RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma

Authors - Hui Han
View Article
humanWB

J Clin Invest

CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes

Authors - Nunziata Maio
View Article