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验证数据展示

  • WB analysis of A549 using 11091-1-AP (same clone as 11091-1-PBS)

    WB analysis of A549 using 11091-1-AP (same clone as 11091-1-PBS)

    WB result of EMAP II antibody (11091-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMAP II transfected A549 cells. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • WB analysis using 11091-1-AP (same clone as 11091-1-PBS)

    WB analysis using 11091-1-AP (same clone as 11091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11091-1-AP (EMAP II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • IP experiment of SGC-7901 using 11091-1-AP (same clone as 11091-1-PBS)

    IP experiment of SGC-7901 using 11091-1-AP (same clone as 11091-1-PBS)

    IP result of anti-EMAP II (IP:11091-1-AP, 4ug; Detection:11091-1-AP 1:500) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

    IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIMP1 antibody included
  • IF Staining of HeLa using 11091-1-AP (same clone as 11091-1-PBS)

    IF Staining of HeLa using 11091-1-AP (same clone as 11091-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EMAP II antibody (11091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EMAP II Polyclonal antibody, PBS Only

EMAP II Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11091-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AIMP1 Ready-To-Use IHC Kit. AIMP1 primary antibody included.

产品信息

11091-1-PBS targets EMAP II in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1560

Product name: Recombinant human SCYE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-312 aa of BC014051

Sequence: MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
别名
计算分子量 34 kDa
观测分子量 34 kDa and 68 kDa
GenBank蛋白编号BC014051
基因名称 EMAP II
Gene ID (NCBI) 9255
RRIDAB_2182590
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ12904
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

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