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EMB Polyclonal antibody

EMB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13946-1-AP

Print datasheet

Synonyms

EMB, Embigin, embigin homolog (mouse)

验证数据展示

  • IHC staining of mouse testis using 13946-1-AP

    IHC staining of mouse testis using 13946-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 13946-1-AP

    WB analysis of HeLa using 13946-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, mouse skeletal muscle tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13946-1-AP targets EMB in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen EMB fusion protein Ag5031 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name embigin homolog (mouse)
Synonyms EMB, Embigin, embigin homolog (mouse)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC059398
Gene Symbol EMB
Gene ID (NCBI) 133418
RRIDAB_2877993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PCB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)

实验方案

Product Specific Protocols
WB protocol for EMB antibody 13946-1-APDownload protocol
IHC protocol for EMB antibody 13946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols