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EML2 Polyclonal antibody

EML2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13529-1-AP

Print datasheet

Synonyms

ELP70, EMAP 2, EMAP2, EMAPL2, EML2, HuEMAP 2

验证数据展示

  • WB analysis of mouse heart using 13529-1-AP

    WB analysis of mouse heart using 13529-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13529-1-AP (EML2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13529-1-AP

    IHC staining of human prostate cancer using 13529-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human prostate cancer using 13529-1-AP

    IHC staining of human prostate cancer using 13529-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse stomach tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13529-1-AP targets EML2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen EML2 fusion protein Ag4423 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name echinoderm microtubule associated protein like 2
Synonyms ELP70, EMAP 2, EMAP2, EMAPL2, EML2, HuEMAP 2
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032630
Gene Symbol EML2
Gene ID (NCBI) 24139
RRIDAB_2098055
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95834
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EML2 antibody 13529-1-APDownload protocol
IHC protocol for EML2 antibody 13529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Hum Genet

MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.

Authors - Sandeep Aryal
View Article
humanWB,IF

Curr Biol

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules

Authors - Takashi Hotta
View Article