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验证数据展示

  • WB analysis of mouse heart using 13529-1-AP

    WB analysis of mouse heart using 13529-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13529-1-AP (EML2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 13529-1-AP

    IHC staining of human prostate cancer using 13529-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human prostate cancer using 13529-1-AP

    IHC staining of human prostate cancer using 13529-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13529-1-AP (EML2 antibody) at dilution of 1:100 (under 40x lens).

EML2 Polyclonal antibody

EML2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13529-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

ELP70, EMAP 2, EMAP2, EMAPL2, EML2, HuEMAP 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse stomach tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13529-1-AP targets EML2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 EML2 fusion protein Ag4423 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 echinoderm microtubule associated protein like 2
别名 ELP70, EMAP 2, EMAP2, EMAPL2, EML2, HuEMAP 2
计算分子量 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC032630
基因名称 EML2
Gene ID (NCBI) 24139
RRIDAB_2098055
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95834
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EML2 antibody 13529-1-APDownload protocol
IHC protocol for EML2 antibody 13529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Hum Genet

MS/MS in silico subtraction-based proteomic profiling as an approach to facilitate disease gene discovery: application to lens development and cataract.

Authors - Sandeep Aryal
View Article
humanWB,IF

Curr Biol

EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules

Authors - Takashi Hotta
View Article