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EML4 Polyclonal antibody

EML4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22709-1-AP

Print datasheet

Synonyms

Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2

验证数据展示

  • WB analysis of A549 using 22709-1-AP

    WB analysis of A549 using 22709-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22709-1-AP

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22709-1-AP

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A549 using 22709-1-AP

    IP experiment of A549 using 22709-1-AP

    IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

  • IF Staining of A549 using 22709-1-AP

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EML4 antibody (22709-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22709-1-AP targets EML4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen EML4 fusion protein Ag18463 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name echinoderm microtubule associated protein like 4
Synonyms Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2
Calculated Molecular Weight 981 aa, 109 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC140845
Gene Symbol EML4
Gene ID (NCBI) 27436
RRIDAB_2879153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9HC35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EML4 antibody 22709-1-APDownload protocol
IHC protocol for EML4 antibody 22709-1-APDownload protocol
IF protocol for EML4 antibody 22709-1-APDownload protocol
IP protocol for EML4 antibody 22709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols