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验证数据展示

  • WB analysis of A549 using 22709-1-AP

    WB analysis of A549 using 22709-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 22709-1-AP

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 22709-1-AP

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of A549 using 22709-1-AP

    IP experiment of A549 using 22709-1-AP

    IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

  • IF Staining of A549 using 22709-1-AP

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EML4 antibody (22709-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

EML4 Polyclonal antibody

EML4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22709-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, A431 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22709-1-AP targets EML4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 EML4 fusion protein Ag18463 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 echinoderm microtubule associated protein like 4
别名 Restrictedly overexpressed proliferation-associated protein, EMAPL4, Echinoderm microtubule-associated protein-like 4, C2orf2
计算分子量 981 aa, 109 kDa
观测分子量 120 kDa
GenBank蛋白编号BC140845
基因名称 EML4
Gene ID (NCBI) 27436
RRIDAB_2879153
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9HC35
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EML4 antibody 22709-1-APDownload protocol
IHC protocol for EML4 antibody 22709-1-APDownload protocol
IF protocol for EML4 antibody 22709-1-APDownload protocol
IP protocol for EML4 antibody 22709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols