• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of U-251 using 26421-1-AP

    WB analysis of U-251 using 26421-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 26421-1-AP

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 26421-1-AP

    IHC staining of human breast cancer using 26421-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26421-1-AP (ENAH Antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 26421-1-AP

    WB analysis using 26421-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26421-1-AP (ENAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 26421-1-AP

    IF Staining of MCF-7 using 26421-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ENAH antibody (26421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ENAH Polyclonal antibody

ENAH Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26421-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ENA, enabled homolog (Drosophila), ENAH, MENA, NDPP1, Protein enabled homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, U-251 cells, MCF-7 cells, HUVEC cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26421-1-AP targets ENAH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 ENAH fusion protein Ag24200 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enabled homolog (Drosophila)
别名 ENA, enabled homolog (Drosophila), ENAH, MENA, NDPP1, Protein enabled homolog
计算分子量 591 aa, 67 kDa
观测分子量 85-88 kDa, 75-80 kDa
GenBank蛋白编号BC095481
基因名称 ENAH
Gene ID (NCBI) 55740
RRIDAB_2880509
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N8S7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ENAH antibody 26421-1-APDownload protocol
IHC protocol for ENAH antibody 26421-1-APDownload protocol
IF protocol for ENAH antibody 26421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Mol Biol Lett

Upregulation of ENAH by a PI3K/AKT/β-catenin cascade promotes oral cancer cell migration and growth via an ITGB5/Src axis

Authors - Xiu-Ya Chan
View Article