验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, Neuro-2a cells, MCF-7 cells, mouse brain tissue, human lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ENC1 Ready-To-Use IHC Kit. ENC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15007-1-AP targets ENC1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | ENC1 fusion protein Ag6607 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ectodermal-neural cortex (with BTB-like domain) |
| 别名 | CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10 |
| 计算分子量 | 66 kDa |
| 观测分子量 | 67 kDa, 57 kDa |
| GenBank蛋白编号 | BC000418 |
| 基因名称 | ENC1 |
| Gene ID (NCBI) | 8507 |
| RRID | AB_2098751 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14682 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENC1 antibody 15007-1-AP | Download protocol |
| IHC protocol for ENC1 antibody 15007-1-AP | Download protocol |
| IP protocol for ENC1 antibody 15007-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.
|
