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  • Featured Product
  • KD/KO Validated

ENC1 Polyclonal antibody

ENC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15007-1-AP

Print datasheet

Synonyms

CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10

验证数据展示

  • WB analysis of SH-SY5Y using 15007-1-AP

    WB analysis of SH-SY5Y using 15007-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15007-1-AP (ENC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 15007-1-AP

    IHC staining of human pancreas using 15007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ENC1 antibody included
  • IHC staining of human pancreas using 15007-1-AP

    IHC staining of human pancreas using 15007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 15007-1-AP (ENC1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ENC1 antibody included
  • IP experiment of mouse brain using 15007-1-AP

    IP experiment of mouse brain using 15007-1-AP

    IP result of anti-ENC1 (IP:15007-1-AP, 4ug; Detection:15007-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

经过测试的应用

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, MCF-7 cells, mouse brain tissue, human lung tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ENC1 Ready-To-Use IHC Kit. ENC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15007-1-AP targets ENC1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ENC1 fusion protein Ag6607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectodermal-neural cortex (with BTB-like domain)
Synonyms CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC000418
Gene Symbol ENC1
Gene ID (NCBI) 8507
RRIDAB_2098751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14682
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ENC1 antibody 15007-1-APDownload protocol
IHC protocol for ENC1 antibody 15007-1-APDownload protocol
IP protocol for ENC1 antibody 15007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Int J Mol Sci

APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.

Authors - Marianna Naki
View Article
humanWB, IHC

Front Cell Dev Biol

ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.

Authors - Ying Cui
  • KD Validated
View Article