验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, Neuro-2a cells, MCF-7 cells, mouse brain tissue, human lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ENC1 Ready-To-Use IHC Kit. ENC1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15007-1-AP targets ENC1 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | ENC1 fusion protein Ag6607 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ectodermal-neural cortex (with BTB-like domain) |
| Synonyms | CCL28, ENC 1, ENC1, Kelch like protein 37, KLHL35, KLHL37, NRPB, Nuclear matrix protein NRP/B, p53 induced gene 10 protein, PIG10, TP53I10 |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 67 kDa, 57 kDa |
| GenBank Accession Number | BC000418 |
| Gene Symbol | ENC1 |
| Gene ID (NCBI) | 8507 |
| RRID | AB_2098751 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14682 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ENC1 antibody 15007-1-AP | Download protocol |
| IHC protocol for ENC1 antibody 15007-1-AP | Download protocol |
| IP protocol for ENC1 antibody 15007-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol ENC1 Facilitates Colorectal Carcinoma Tumorigenesis and Metastasis via JAK2/STAT5/AKT Axis-Mediated Epithelial Mesenchymal Transition and Stemness.
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