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  • KD/KO Validated

Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10862-1-AP

Print datasheet

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1



经过测试的应用

Positive WB detected inHUVEC cells, human placenta tissue, mouse lung tissue, rat lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, rat kidney tissue, human placenta tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD105 Ready-To-Use IHC Kit. CD105 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10862-1-AP targets Endoglin/CD105 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Immunogen Endoglin/CD105 fusion protein Ag1287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endoglin
Synonyms CD105, END, Endoglin, ENG, HHT1, ORW, ORW1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 75-90 kDa, 170-180 kDa
GenBank Accession NumberBC014271
Gene Symbol CD105
Gene ID (NCBI) 2022
RRIDAB_2098906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
IP protocol for Endoglin/CD105 antibody 10862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Dev Cell

Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation

Authors - Bin Tan
humanWB

Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand
humanWB

Int J Biol Sci

Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.

Authors - Meicen Zhou
humanWB

Biomedicines

Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.

Authors - Mahendra Jadhao
  • KD Validated
humanIHC

Mol Hum Reprod

Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells.

Authors - Tskitishvili E E
ratIF

World J Stem Cells

Quercetin ameliorates oxidative stress-induced senescence in rat nucleus pulposus-derived mesenchymal stem cells via the miR-34a-5p/SIRT1 axis

Authors - Wen-Jie Zhao