验证数据展示
经过测试的应用
Positive WB detected in | HUVEC cells, human placenta tissue, mouse lung tissue, rat lung tissue |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | human breast cancer tissue, rat kidney tissue, human placenta tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10862-1-AP targets Endoglin/CD105 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, bovine |
免疫原 | Endoglin/CD105 fusion protein Ag1287 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | endoglin |
别名 | CD105, END, Endoglin, ENG, HHT1 |
计算分子量 | 71 kDa |
观测分子量 | 75-90 kDa, 170-180 kDa |
GenBank蛋白编号 | BC014271 |
基因名称 | CD105 |
Gene ID (NCBI) | 2022 |
RRID | AB_2098906 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P17813 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)
实验方案
Product Specific Protocols | |
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WB protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
IHC protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
IP protocol for Endoglin/CD105 antibody 10862-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Dev Cell Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation | ||
Stem Cell Res Ther Controlled release of hydrogel-encapsulated mesenchymal stem cells-conditioned medium promotes functional liver regeneration after hepatectomy in metabolic dysfunction-associated steatotic liver disease | ||
Cancers (Basel) WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells. | ||
Int J Biol Sci Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA. | ||
Biomedicines Endoglin Modulates TGFβR2 Induced VEGF and Proinflammatory Cytokine Axis Mediated Angiogenesis in Prolonged DEHP-Exposed Breast Cancer Cells.
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Mol Hum Reprod Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells. |