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验证数据展示

  • WB analysis using 81504-1-RR (same clone as 81504-1-PBS)

    WB analysis using 81504-1-RR (same clone as 81504-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81504-1-RR (same clone as 81504-1-PBS)

    WB analysis of HEK-293 using 81504-1-RR (same clone as 81504-1-PBS)

    WB result of ENO1/2/3 antibody (81504-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1/2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81504-1-RR (same clone as 81504-1-PBS)

    IF Staining of HepG2 using 81504-1-RR (same clone as 81504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1/2/3 antibody (81504-1-RR, Clone: 6E24 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ENO1/2/3 Recombinant antibody, PBS Only

ENO1/2/3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81504-1-PBS

产品说明书

CloneNo. 6E24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

产品信息

81504-1-PBS targets ENO1/2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ENO1/2/3 fusion protein Ag1692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enolase 1, (alpha)
别名 ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC015641
基因名称 ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06733
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.