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  • Featured Product
  • KD/KO Validated

ENO1/2/3 Recombinant antibody, PBS Only

ENO1/2/3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6E24

Cat no : 81504-1-PBS

Print datasheet

Synonyms

ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24

验证数据展示

  • WB analysis using 81504-1-RR (same clone as 81504-1-PBS)

    WB analysis using 81504-1-RR (same clone as 81504-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81504-1-RR (same clone as 81504-1-PBS)

    WB analysis of HEK-293 using 81504-1-RR (same clone as 81504-1-PBS)

    WB result of ENO1/2/3 antibody (81504-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1/2/3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    IHC staining of mouse brain using 81504-1-RR (same clone as 81504-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81504-1-RR (ENO1/2/3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81504-1-RR (same clone as 81504-1-PBS)

    IF Staining of HepG2 using 81504-1-RR (same clone as 81504-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ENO1/2/3 antibody (81504-1-RR, Clone: 6E24 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81504-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

产品信息

81504-1-PBS targets ENO1/2/3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ENO1/2/3 fusion protein Ag1692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enolase 1, (alpha)
Synonyms ENO1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 6E24
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06733
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enolase (ENO), also known as 2-phospho-D-glycerate hydrolase, is a metalloenzyme that catalyzes the dehydration of 2-phospho-D-glycerate into phosphoenolpyruvate in the glycolytic pathway. There are three different ENO isoforms in higher eukaryotes, including ENO1 or α-ENO, ENO2 or γ-ENO, and ENO3 or β-ENO. Furthermore, the expression of the different ENO isoforms is tissue specificity. ENO1 is expressed in almost all kinds of tissue, whereas ENO3 is restricted to muscle tissues and ENO2 is described as a neuron- and neuroendocrine tissue-specific enolase. ENO1 serves multifunctional roles in physiological and pathological processes. (PMID: 32256815). This antibody can recognize ENO1, ENO2, and ENO3.