• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ENO1 Recombinant antibody, PBS Only

ENO1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C20

Cat no : 81478-1-PBS

Print datasheet

Synonyms

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

验证数据展示

  • WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

    IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (81478-1-RR, Clone: 2C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81478-1-RR

    Affinity and Kinetic Characterization of 81478-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81478-1-RR against Human ENO1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

产品信息

81478-1-PBS targets ENO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ENO1 fusion protein Ag1692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enolase 1, (alpha)
Synonyms ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06733
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.