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Species Reactivity
0
Species Reactivity
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    WB analysis using 81478-1-RR (same clone as 81478-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81478-1-RR (ENO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81478-1-RR (same clone as 81478-1-PBS)

    WB analysis of HEK-293 using 81478-1-RR (same clone as 81478-1-PBS)

    WB result of ENO1 antibody (81478-1-RR; 1:300000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    IHC staining of mouse brain using 81478-1-RR (same clone as 81478-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81478-1-RR (ENO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ENO1 antibody included
  • IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

    IF Staining of HepG2 using 81478-1-RR (same clone as 81478-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (81478-1-RR, Clone: 2C20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81478-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81478-1-RR

    Affinity and Kinetic Characterization of 81478-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81478-1-RR against Human ENO1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ENO1 Recombinant antibody, PBS Only

ENO1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81478-1-PBS

产品说明书

CloneNo. 2C20

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

产品信息

81478-1-PBS targets ENO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ENO1 fusion protein Ag1692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enolase 1, (alpha)
别名 ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC015641
基因名称 ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP06733
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross-reaction with ENO2 and ENO3.