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NSE/ENO2-specific Polyclonal antibody

NSE/ENO2-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55235-1-AP

Print datasheet

Synonyms

Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific

验证数据展示

  • WB analysis of SH-SY5Y using 55235-1-AP

    WB analysis of SH-SY5Y using 55235-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55235-1-AP (NSE/ENO2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of U-251 using 55235-1-AP

    WB analysis of U-251 using 55235-1-AP

    WB result of NSE/ENO2-specific antibody (55235-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSE/ENO2-specific transfected U-251 cells.

  • IHC staining of human brain using 55235-1-AP

    IHC staining of human brain using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 10x lens).

  • IHC staining of human brain using 55235-1-AP

    IHC staining of human brain using 55235-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55235-1-AP (NSE/ENO2-specific antibody at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse brain tissue, HEK-293 cells, HepG2 cells, U-251 cells
Positive IHC detected inhuman brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

55235-1-AP targets NSE/ENO2-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase 2 (gamma, neuronal)
Synonyms Enolase 2, enolase 2 (gamma, neuronal), Gamma enolase, Neural enolase, Neuron specific enolase, NSE, NSE/ENO2-specific
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberNM_001975
Gene Symbol NSE
Gene ID (NCBI) 2026
RRIDAB_10863290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

NSE, also named as ENO2, belongs to the enolase family. Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors (PMID:7520111). ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. This antibody is specific to ENO2 and has no cross reaction to ENO1 and ENO3.

实验方案

Product Specific Protocols
WB protocol for NSE/ENO2-specific antibody 55235-1-APDownload protocol
IHC protocol for NSE/ENO2-specific antibody 55235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Med Rep

miR‑153 promotes neural differentiation in the mouse hippocampal HT‑22 cell line and increases the expression of neuron‑specific enolase.

Authors - Chunli Xu
View Article