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- KD/KO Validated
ENO3-specific Polyclonal antibody
ENO3-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, SH-SY5Y cells, HepG2 cells, mouse brain tissue, Jurkat cells |
Positive IHC detected in | mouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
产品信息
55234-1-AP targets ENO3-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | enolase 3 (beta, muscle) |
Synonyms | Beta enolase, ENO3, ENO3-specific, Enolase 3, enolase 3 (beta, muscle), MSE, Muscle specific enolase, Skeletal muscle enolase |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 42-47 kDa |
GenBank Accession Number | NM_001976 |
Gene Symbol | ENO3 |
Gene ID (NCBI) | 2027 |
RRID | AB_10837363 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P13929 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
IHC protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
IF protocol for ENO3-specific antibody 55234-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Proteomics Characterization of the liver proteome in dairy cows experiencing negative energy balance at early lactation. | ||
Ann Transl Med ENO3 promoted the progression of NASH by negatively regulating ferroptosis via elevation of GPX4 expression and lipid accumulation.
| ||
Med Oncol ENO3 promotes colorectal cancer progression by enhancing cell glycolysis.
| ||
Reprod Domest Anim Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation. |