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验证数据展示

  • WB analysis using 31022-1-AP

    WB analysis using 31022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 31022-1-AP

    IP experiment of MCF-7 using 31022-1-AP

    IP result of anti-ENOSF1 (IP:31022-1-AP, 4ug; Detection:31022-1-AP 1:2000) with MCF-7 cells lysate 1600 ug.

  • FC experiment of HeLa using 31022-1-AP

    FC experiment of HeLa using 31022-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ENOSF1 Polyclonal antibody (31022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ENOSF1 Polyclonal antibody

ENOSF1 Polyclonal Antibody for WB, FC (Intra), IP, ELISA
Cat No. 31022-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, ELISA

别名

EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31022-1-AP targets ENOSF1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 ENOSF1 fusion protein Ag34665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enolase superfamily member 1
别名 EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS
计算分子量 50 kDa
观测分子量 49 kDa
GenBank蛋白编号BC001285
基因名称 ENOSF1
Gene ID (NCBI) 55556
RRIDAB_3669818
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7L5Y1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

实验方案

Product Specific Protocols
WB protocol for ENOSF1 antibody 31022-1-APDownload protocol
IP protocol for ENOSF1 antibody 31022-1-APDownload protocol
FC protocol for ENOSF1 antibody 31022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols