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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HuH-7 using 68724-1-Ig (same clone as 68724-1-PBS)

    WB analysis of HuH-7 using 68724-1-Ig (same clone as 68724-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • WB analysis of U-87 MG using 68724-1-Ig (same clone as 68724-1-PBS)

    WB analysis of U-87 MG using 68724-1-Ig (same clone as 68724-1-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

  • WB analysis using 68724-1-Ig (same clone as 68724-1-PBS)

    WB analysis using 68724-1-Ig (same clone as 68724-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68724-1-Ig (ENPP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68724-1-PBS in a different storage buffer formulation.

ENPP2 Monoclonal antibody, PBS Only

ENPP2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68724-1-PBS

产品说明书

CloneNo. 2A10D9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, Indirect ELISA

别名

AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68724-1-PBS targets ENPP2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 ENPP2 fusion protein Ag5499 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ectonucleotide pyrophosphatase/phosphodiesterase 2
别名 AUTOTAXIN, E-NPP 2, EC:3.1.4.39, E NPP 2, ATX X
计算分子量 99 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC034961
基因名称 ENPP2
Gene ID (NCBI) 5168
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13822
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ENPP2, Autotaxin or Extracellular lysophospholipase D, is a 863 amino acid secreted protein, which is detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). ENPP2 is Predominantly expressed in brain, placenta, ovary, and small intestine. ENPP2 is expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. ENPP2 is expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. The calculated molecular weight of ENPP2 is about 100 kDa (PMID: 22301782), but modified ENPP2 is 120 kDa (PMID: 19808661). ENPP2 Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931).