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验证数据展示

  • WB analysis of HL-60 using 17305-1-AP

    WB analysis of HL-60 using 17305-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17305-1-AP (ENPP4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17305-1-AP

    IHC staining of human brain using 17305-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17305-1-AP

    IHC staining of human brain using 17305-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 40x lens).

ENPP4 Polyclonal antibody

ENPP4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17305-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E NPP 4, ENPP4, KIAA0879

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17305-1-AP targets ENPP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ENPP4 fusion protein Ag11192 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative function)
别名 E NPP 4, ENPP4, KIAA0879
计算分子量 453 aa, 52 kDa
观测分子量 52-60 kDa
GenBank蛋白编号BC018054
基因名称 ENPP4
Gene ID (NCBI) 22875
RRIDAB_2099780
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6X5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENPP4, also named as KIAA0879 or NPP4, is a 453 amino acid protein, which belongs to the nucleotide pyrophosphatase/phosphodiesterase family. ENPP4 localizes in the cell membrane and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. ENPP4 is expressed on the surface of vascular endothelia.

实验方案

Product Specific Protocols
WB protocol for ENPP4 antibody 17305-1-APDownload protocol
IHC protocol for ENPP4 antibody 17305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Blood

NPP4 is a procoagulant enzyme on the surface of vascular endothelium.

Authors - Albright Ronald A RA
View Article