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验证数据展示

  • WB analysis of MCF-7 using 12778-1-AP

    WB analysis of MCF-7 using 12778-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12778-1-AP (ENPP5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12778-1-AP

    IHC staining of human gliomas using 12778-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12778-1-AP (ENPP5 antibody) at dilution of 1:100 (under 10x lens).

ENPP5 Polyclonal antibody

ENPP5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12778-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

E NPP 5, ENPP5, KIAA0879

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12778-1-AP targets ENPP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ENPP5 fusion protein Ag3478 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative function)
别名 E NPP 5, ENPP5, KIAA0879
计算分子量 477 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC027615
基因名称 ENPP5
Gene ID (NCBI) 59084
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJA9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.