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ENSA Polyclonal antibody

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14518-1-AP

Print datasheet

Synonyms

ARPP-19e, Alpha-endosulfine

验证数据展示

  • WB analysis of mouse brain using 14518-1-AP

    WB analysis of mouse brain using 14518-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 14518-1-AP

    WB analysis using 14518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 14518-1-AP

    IHC staining of human small intestine using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 14518-1-AP

    IF Staining of HeLa using 14518-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14518-1-AP (ENSA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 14518-1-AP

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 14518-1-AP

    IHC staining of human colon using 14518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, COLO 320 cells, HeLa cells
Positive IHC detected inhuman small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14518-1-AP targets ENSA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ENSA fusion protein Ag5982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name endosulfine alpha
Synonyms ARPP-19e, Alpha-endosulfine
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC004461
Gene Symbol ENSA
Gene ID (NCBI) 2029
RRIDAB_10838808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43768
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.

实验方案

Product Specific Protocols
WB protocol for ENSA antibody 14518-1-APDownload protocol
IHC protocol for ENSA antibody 14518-1-APDownload protocol
IF protocol for ENSA antibody 14518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols