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验证数据展示

  • WB analysis of human placenta using 13656-1-AP

    WB analysis of human placenta using 13656-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13656-1-AP (ENTPD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 13656-1-AP

    IHC staining of human small intestine using 13656-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human small intestine using 13656-1-AP

    IHC staining of human small intestine using 13656-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13656-1-AP (ENTPD2 antibody) at dilution of 1:200 (under 40x lens).

  • IP experiment of K-562 using 13656-1-AP

    IP experiment of K-562 using 13656-1-AP

    IP result of anti-ENTPD2 (IP:13656-1-AP, 4ug; Detection:13656-1-AP 1:300) with K-562 cells lysate 4000ug.

ENTPD2 Polyclonal antibody

ENTPD2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13656-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IP, IHC, ELISA

别名

CD39 antigen like 1, CD39L1, Ecto ATP diphosphohydrolase 2, Ecto ATPase 2, Ecto ATPDase 2, ENTPD2, NTPDase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

13656-1-AP targets ENTPD2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性rat
免疫原 ENTPD2 fusion protein Ag4513 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleoside triphosphate diphosphohydrolase 2
别名 CD39 antigen like 1, CD39L1, Ecto ATP diphosphohydrolase 2, Ecto ATPase 2, Ecto ATPDase 2, ENTPD2, NTPDase 2
计算分子量 495 aa, 54 kDa
观测分子量 70 kDa
GenBank蛋白编号BC035738
基因名称 ENTPD2
Gene ID (NCBI) 954
RRIDAB_2877966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5L3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD2(Ectonucleoside triphosphate diphosphohydrolase 2) is also named as CD39L1, Ecto-ATPase 2 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ENTPD2 antibody 13656-1-APDownload protocol
IHC protocol for ENTPD2 antibody 13656-1-APDownload protocol
IP protocol for ENTPD2 antibody 13656-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Am J Physiol Gastrointest Liver Physiol

Water intake accelerates ATP release from myofibroblast cells in rats: ATP-mediated podoplanin-dependent control for physiological function and immunity.

Authors - Moyuru Hayashi
View Article